O21.6 A Tale of Two Cities: Treponema Pallidum Macrolide Resistance in Colombo (Sri Lanka) and London (United Kingdom)
Identifieur interne : 000663 ( Main/Exploration ); précédent : 000662; suivant : 000664O21.6 A Tale of Two Cities: Treponema Pallidum Macrolide Resistance in Colombo (Sri Lanka) and London (United Kingdom)
Auteurs : D. Mallikarachchi [Sri Lanka] ; L. Hodson [Royaume-Uni] ; C. Duckett [Royaume-Uni] ; G. Weerasinghe [Sri Lanka] ; K. Buddhakorale [Sri Lanka] ; M. Mcclure [Royaume-Uni] ; G. Taylor [Royaume-Uni] ; C. Tipple [Royaume-Uni]Source :
- Sexually Transmitted Infections [ 1368-4973 ] ; 2013-07.
Descripteurs français
- Wicri :
- topic : Antibiotique, Sage-femme.
English descriptors
- KwdEn :
- Teeft :
Abstract
Background The bacterium Treponema pallidum ( T. pallidum) causes syphilis. Penicillin is effective treatment, but azithromycin (a macrolide) is a single-dose oral alternative for those with allergy. Unfortunately, macrolide resistance secondary to one of two 23S ribosomal RNA (rRNA) point mutations (A2058G and A2059G) is now wide-spread. Molecular strain-typing suggests that epidemics and macrolide resistance are unlikely the spread of single clones. We present typing and macrolide resistance data from two geographically distinct populations: Colombo, Sri Lanka and London, UK. Methods Cross-sectional studies were conducted at the Colombo District STD clinics and St Mary’s Hospital, London. Ulcer exudate and/or blood were collected from patients with microbiologically confirmed syphilis. Presence of T. pallidum DNA ( tpp047 gene) was confirmed with PCR. Next, using published techniques, the 23SrRNAgene was PCR-amplified for a point-mutation assay and tpp0548, arp and tprE,G& Jamplicons were used for strain-typing. Results Sri Lanka: 24 T. pallidum PCR-positive samples were collected. Patients were men (45.9% MSM) and 91.6% Sinhalese with a mean age of 28 (range 29). None were HIV-1 infected. Two strain types were discovered (14b/f and 13b/f), neither harbouring macrolide resistance. London: 43 men were recruited, 18 in 2006–8 and 25 in 2011–12. Mean age was 37.5 (range 43); 95.2% were MSM and 62.8% were HIV-1 infected. Half (22/43) were white British. A total of 5 full and 14 partial strain types were identified, of which 6 were unique. Macrolide resistance increased from 66.7%(12/18) in 2006–8 to 80%(20/25) in 2011–12. Conclusion Colombo T. pallidumstrains have limited diversity with no macrolide resistance. London strains are more varied and increasingly macrolide-resistant. Ethnic diversity in London exceeds Colombo’s and may explain increased strain diversity. In contrast to Sri Lanka, azithromycin is widely used to treat Chlamydia and non-specific urethritis in the UK thus selection pressure may be driving macrolide resistance.
Url:
DOI: 10.1136/sextrans-2013-051184.0208
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000623
- to stream Istex, to step Curation: 000623
- to stream Istex, to step Checkpoint: 000028
- to stream Main, to step Merge: 000664
- to stream Main, to step Curation: 000663
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">O21.6 A Tale of Two Cities: Treponema Pallidum Macrolide Resistance in Colombo (Sri Lanka) and London (United Kingdom)</title><author><name sortKey="Mallikarachchi, D" sort="Mallikarachchi, D" uniqKey="Mallikarachchi D" first="D" last="Mallikarachchi">D. Mallikarachchi</name></author><author><name sortKey="Hodson, L" sort="Hodson, L" uniqKey="Hodson L" first="L" last="Hodson">L. Hodson</name></author><author><name sortKey="Duckett, C" sort="Duckett, C" uniqKey="Duckett C" first="C" last="Duckett">C. Duckett</name></author><author><name sortKey="Weerasinghe, G" sort="Weerasinghe, G" uniqKey="Weerasinghe G" first="G" last="Weerasinghe">G. Weerasinghe</name></author><author><name sortKey="Buddhakorale, K" sort="Buddhakorale, K" uniqKey="Buddhakorale K" first="K" last="Buddhakorale">K. Buddhakorale</name></author><author><name sortKey="Mcclure, M" sort="Mcclure, M" uniqKey="Mcclure M" first="M" last="Mcclure">M. Mcclure</name></author><author><name sortKey="Taylor, G" sort="Taylor, G" uniqKey="Taylor G" first="G" last="Taylor">G. Taylor</name></author><author><name sortKey="Tipple, C" sort="Tipple, C" uniqKey="Tipple C" first="C" last="Tipple">C. Tipple</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:8E4C004D62B8DA37B3DB67788B37960128AC9E49</idno><date when="2013" year="2013">2013</date><idno type="doi">10.1136/sextrans-2013-051184.0208</idno><idno type="url">https://api.istex.fr/document/8E4C004D62B8DA37B3DB67788B37960128AC9E49/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000623</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000623</idno><idno type="wicri:Area/Istex/Curation">000623</idno><idno type="wicri:Area/Istex/Checkpoint">000028</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000028</idno><idno type="wicri:doubleKey">1368-4973:2013:Mallikarachchi D:o:a:tale</idno><idno type="wicri:Area/Main/Merge">000664</idno><idno type="wicri:Area/Main/Curation">000663</idno><idno type="wicri:Area/Main/Exploration">000663</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">O21.6 A Tale of Two Cities: Treponema Pallidum Macrolide Resistance in Colombo (Sri Lanka) and London (United Kingdom)</title><author><name sortKey="Mallikarachchi, D" sort="Mallikarachchi, D" uniqKey="Mallikarachchi D" first="D" last="Mallikarachchi">D. Mallikarachchi</name><affiliation wicri:level="1"><country xml:lang="fr">Sri Lanka</country><wicri:regionArea>National Centre for STD/AIDS Control, Colombo</wicri:regionArea><wicri:noRegion>Colombo</wicri:noRegion></affiliation></author><author><name sortKey="Hodson, L" sort="Hodson, L" uniqKey="Hodson L" first="L" last="Hodson">L. Hodson</name><affiliation wicri:level="3"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Imperial College Healthcare NHS Trust, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author><author><name sortKey="Duckett, C" sort="Duckett, C" uniqKey="Duckett C" first="C" last="Duckett">C. Duckett</name><affiliation wicri:level="3"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Imperial College Healthcare NHS Trust, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author><author><name sortKey="Weerasinghe, G" sort="Weerasinghe, G" uniqKey="Weerasinghe G" first="G" last="Weerasinghe">G. Weerasinghe</name><affiliation wicri:level="1"><country xml:lang="fr">Sri Lanka</country><wicri:regionArea>National Centre for STD/AIDS Control, Colombo</wicri:regionArea><wicri:noRegion>Colombo</wicri:noRegion></affiliation></author><author><name sortKey="Buddhakorale, K" sort="Buddhakorale, K" uniqKey="Buddhakorale K" first="K" last="Buddhakorale">K. Buddhakorale</name><affiliation wicri:level="1"><country xml:lang="fr">Sri Lanka</country><wicri:regionArea>National Centre for STD/AIDS Control, Colombo</wicri:regionArea><wicri:noRegion>Colombo</wicri:noRegion></affiliation></author><author><name sortKey="Mcclure, M" sort="Mcclure, M" uniqKey="Mcclure M" first="M" last="Mcclure">M. Mcclure</name><affiliation wicri:level="3"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Imperial College London, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author><author><name sortKey="Taylor, G" sort="Taylor, G" uniqKey="Taylor G" first="G" last="Taylor">G. Taylor</name><affiliation wicri:level="3"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Imperial College London, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author><author><name sortKey="Tipple, C" sort="Tipple, C" uniqKey="Tipple C" first="C" last="Tipple">C. Tipple</name><affiliation wicri:level="3"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Imperial College London, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Sexually Transmitted Infections</title><title level="j" type="abbrev">Sex Transm Infect</title><idno type="ISSN">1368-4973</idno><idno type="eISSN">1472-3263</idno><imprint><publisher>BMJ Publishing Group Ltd</publisher><date type="published" when="2013-07">2013-07</date><biblScope unit="volume">89</biblScope><biblScope unit="issue">Suppl 1</biblScope><biblScope unit="page" from="A68">A68</biblScope></imprint><idno type="ISSN">1368-4973</idno></series><idno type="istex">8E4C004D62B8DA37B3DB67788B37960128AC9E49</idno><idno type="DOI">10.1136/sextrans-2013-051184.0208</idno><idno type="href">sextrans-89-A68-2.pdf</idno><idno type="ArticleID">sextrans-2013-051184.0208</idno><idno type="local">sextrans;89/Suppl_1/A68-b</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1368-4973</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Active syphilis</term><term>Ambedkar center</term><term>Antibiotic</term><term>Antimicrobial resistance</term><term>Biomedical research</term><term>Colombo</term><term>Lanka</term><term>Macrolide</term><term>Macrolide resistance</term><term>Midwife</term><term>Mtrr</term><term>Mutant</term><term>Mutant mtrr</term><term>Mutation</term><term>Pallidum</term><term>Pregnant women</term><term>Syphilis</term><term>Wild type</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Active syphilis</term><term>Ambedkar center</term><term>Antibiotic</term><term>Antimicrobial resistance</term><term>Biomedical research</term><term>Colombo</term><term>Lanka</term><term>Macrolide</term><term>Macrolide resistance</term><term>Midwife</term><term>Mtrr</term><term>Mutant</term><term>Mutant mtrr</term><term>Mutation</term><term>Pallidum</term><term>Pregnant women</term><term>Syphilis</term><term>Wild type</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Antibiotique</term><term>Sage-femme</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">Background The bacterium Treponema pallidum ( T. pallidum) causes syphilis. Penicillin is effective treatment, but azithromycin (a macrolide) is a single-dose oral alternative for those with allergy. Unfortunately, macrolide resistance secondary to one of two 23S ribosomal RNA (rRNA) point mutations (A2058G and A2059G) is now wide-spread. Molecular strain-typing suggests that epidemics and macrolide resistance are unlikely the spread of single clones. We present typing and macrolide resistance data from two geographically distinct populations: Colombo, Sri Lanka and London, UK. Methods Cross-sectional studies were conducted at the Colombo District STD clinics and St Mary’s Hospital, London. Ulcer exudate and/or blood were collected from patients with microbiologically confirmed syphilis. Presence of T. pallidum DNA ( tpp047 gene) was confirmed with PCR. Next, using published techniques, the 23SrRNAgene was PCR-amplified for a point-mutation assay and tpp0548, arp and tprE,G& Jamplicons were used for strain-typing. Results Sri Lanka: 24 T. pallidum PCR-positive samples were collected. Patients were men (45.9% MSM) and 91.6% Sinhalese with a mean age of 28 (range 29). None were HIV-1 infected. Two strain types were discovered (14b/f and 13b/f), neither harbouring macrolide resistance. London: 43 men were recruited, 18 in 2006–8 and 25 in 2011–12. Mean age was 37.5 (range 43); 95.2% were MSM and 62.8% were HIV-1 infected. Half (22/43) were white British. A total of 5 full and 14 partial strain types were identified, of which 6 were unique. Macrolide resistance increased from 66.7%(12/18) in 2006–8 to 80%(20/25) in 2011–12. Conclusion Colombo T. pallidumstrains have limited diversity with no macrolide resistance. London strains are more varied and increasingly macrolide-resistant. Ethnic diversity in London exceeds Colombo’s and may explain increased strain diversity. In contrast to Sri Lanka, azithromycin is widely used to treat Chlamydia and non-specific urethritis in the UK thus selection pressure may be driving macrolide resistance.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li><li>Sri Lanka</li></country><region><li>Angleterre</li><li>Grand Londres</li></region><settlement><li>Londres</li></settlement></list><tree><country name="Sri Lanka"><noRegion><name sortKey="Mallikarachchi, D" sort="Mallikarachchi, D" uniqKey="Mallikarachchi D" first="D" last="Mallikarachchi">D. Mallikarachchi</name></noRegion><name sortKey="Buddhakorale, K" sort="Buddhakorale, K" uniqKey="Buddhakorale K" first="K" last="Buddhakorale">K. Buddhakorale</name><name sortKey="Weerasinghe, G" sort="Weerasinghe, G" uniqKey="Weerasinghe G" first="G" last="Weerasinghe">G. Weerasinghe</name></country><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Hodson, L" sort="Hodson, L" uniqKey="Hodson L" first="L" last="Hodson">L. Hodson</name></region><name sortKey="Duckett, C" sort="Duckett, C" uniqKey="Duckett C" first="C" last="Duckett">C. Duckett</name><name sortKey="Mcclure, M" sort="Mcclure, M" uniqKey="Mcclure M" first="M" last="Mcclure">M. Mcclure</name><name sortKey="Taylor, G" sort="Taylor, G" uniqKey="Taylor G" first="G" last="Taylor">G. Taylor</name><name sortKey="Tipple, C" sort="Tipple, C" uniqKey="Tipple C" first="C" last="Tipple">C. Tipple</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/SidaGhanaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000663 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000663 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= SidaGhanaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:8E4C004D62B8DA37B3DB67788B37960128AC9E49
|texte= O21.6 A Tale of Two Cities: Treponema Pallidum Macrolide Resistance in Colombo (Sri Lanka) and London (United Kingdom)
}}
| This area was generated with Dilib version V0.6.31. |  |